Multiple group I introns in the small-subunit rDNA of Botryosphaeria dothidea: implication for intraspecific genetic diversity.

Multiple group I introns in the small-subunit rDNA of Botryosphaeria dothidea: implication for intraspecific genetic diversity.

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Botryosphaeria dothidea is a widespread and economically important pathogen on various fruit trees, and it often causes die-back and canker on limbs and fruit rot.In characterizing intraspecies genetic variation within this fungus, group I introns, Phone Accessories rich in rDNA of fungi, may provide a productive region for exploration.In this research, we analysed complete small subunit (SSU) ribosomal DNA (rDNA) sequences of 37 B.dothidea strains, and found four insertions, designated Bdo.S943, Bdo.

S1199-A, Bdo.S1199-B and Bdo.S1506, at three positions.Sequence analysis and structure prediction revealed that both Bdo.S943 and Bdo.

S1506 belonged to subgroup IC1 of group I introns, whereas Bdo.S1199-A and Bdo.S1199-B corresponded to group IE introns.Moreover, Bdo.S1199-A was found to host an open reading frame (ORF) for encoding the homing endonuclease (HE), whereas Bdo.

S1199-B, an evolutionary descendant of Bdo.S1199-A, included a degenerate HE.The above four introns were novel, and were the first group I introns observed and characterized in this species.Differential distribution of these Machine Embroidery Accessories introns revealed that all strains could be separated into four genotypes.Genotype III (no intron) and genotype IV (Bdo.

S1199-B) were each found in only one strain, whereas genotype I (Bdo.S1199-A) and genotype II (Bdo.S943 and Bdo.S1506) occurred in 95% of the strains.There is a correlation between B.

dothidea genotypes and hosts or geographic locations.Thus, these newly discovered group I introns can help to advance understanding of genetic differentiation within B.dothidea.